PATENT NR. 129397
Metodă și dispozitiv pentru identificarea simultană a microorganismelor de tip ESCHERICHIA COLI, SALMONELLA SPP., LISTERIA MONOCYTOGENES, CAMPYLOBACTER SPP. din alimente
Abstract: The invention relates to a method and device needed for the rapid identification of biological agents type Escherichia coli, Salmonella spp. , Listeria monocytogenes, Campylobacter spp. from foods.
The method according to the invention consists in quantifying the fluorescence, taking as reference the wavelengths of the characteristic fluorescence spectrum of each microorganism to be observed, establishing a univariate relationship between the fluorescence of the natural amino acids of the target microorganisms and the type of microorganisms that generated it, specific fluorescence being directly proportional to the type and concentration of excited amino acids, the fluorescence spectrum characteristic of each identified microorganism being obtained by excitement with a light source of a conditioned food sample with para-magnetic nanoparticles of Fe3O4,functionalized specifically, directly collected by immunomagnetic separation, in which the paramagnetic nanoparticles of Fe3O4 are coated with an organosilane layer with amine functionality to prevent chemical degradation processes and micromagnetic agglomeration, and subsequently they are functionalized in basic medium with glutaraldehyde, protein A, or specific antibodies Escherichia coli, Salmonella spp., Listeria monocytogenes, Campylobacter spp.
The device according to the invention integrates 5 modules, each of which fulfils different functions:
– a micro-module (MP) directly determines and condition the food samples in view of the biochemical marker;
– a biochemical marker micro-mod (MN), which injected controlled in the first micro-module (MP) nanoparticles functionalized with specific antibody;
– a magnetic separation micro-module (MsM) that integrates a magnetic microprobe for the controlled separation of antibody-labelled biohazards;
– a detection microprobe (OiD) integrating an optical interface designed to integrate and scan the magnetic microprobe, and a micro-module (MCC) command and control.